Halquinol 0.3% cream

AbstractIn this study, an analysis method was established for the quantification of residues of halquinol and its metabolites in livestock and fishery products using liquid chromatography–tandem mass spectrometry (LC–MS/MS). We selected beef, pork (muscle and fat), chicken, egg, milk, flat fish, eel, and shrimp as target samples for validation of the method owing to them being typical livestock and fishery products. Validation of the developed analysis method was performed using liquid chromatography–tandem mass spectrometry (LC–MS/MS) at three concentration levels (0.5, 1, and 2 × the maximum residue limits) following the Codex Alimentarius (CODEX) guidelines (CAC/GL 71–2009). For all samples, correlation coefficients (R2) exceeded 0.99, recoveries ranged between 75.59 and 119.36%, and coefficients of variation (CV) ranged between 1.39 and 28.66%, thus satisfying CODEX guidelines. In addition, inter-laboratory validation was conducted, and the resulting recoveries and CVs satisfied the CODEX guidelines; LOQ was established as 10 μg kg–1 for pig muscle and 5 μg kg–1 for the other samples. Therefore, the analysis method developed in this study can accurately and precisely screen for and quantify halquinol and its metabolites in livestock and fishery products.

Abstract licence: CC BY 4.0

ABSTRACTAntimicrobial resistance is an increasingly pressing global concern, with one of its contributing factors being the use of subtherapeutic doses of antibiotics in animal production for zootechnical purposes. Halquinol (HA) remains one of the few compounds still permitted in certain regions; however, the development of alternative solutions is imperative. This study evaluated the in vitro effects of a formulation composed of essential oils and organic acids against Salmonella and Escherichia coli as well as its interaction with HA. The formulation demonstrated both inhibitory and bactericidal activity against the bacteria, with effective concentrations ranging from 2.0 to 8.0 mL/L. In contrast, HA exhibited solely bacteriostatic effects, with minimum inhibitory concentrations ranging from 37.5 to 300 μg/mL. When used in combination, the compounds predominantly resulted in additive or indifferent interactions: 51.35% of the assays showed additive effects, 43.24% exhibited no interaction, and only 5.41% demonstrated antagonistic interactions. Importantly, the formulation did not induce bacterial resistance or adaptation following sublethal exposure. Conversely, sublethal exposure to HA led to an approximately tenfold increase in its bacteriostatic dose. Moreover, the formulation showed potential to mitigate resistance induced by sublethal HA exposure. In a simulated swine digestion, the combination of the formulation with HA was particularly effective against E. coli, with the co‐administration reducing the required concentrations of the individual compounds to achieve sustained bacterial suppression throughout the digestive process. Based on these findings, the tested formulation demonstrates promising potential for bacterial control and may be used in combination with HA to reduce its required dosage.

Abstract licence: CC BY 4.0

Simple Summary Antimicrobial growth promoters (AGPs) are banned in Europe but still used in many countries including Asia. However, their indiscriminate use resulted in antibiotic-resistant bacterial strains that possibly transfer the resistant genes to the microorganisms pertinent to human health. Hence, it is essential to find alternatives that can improve the production performance in broiler chickens. In this scenario, phytobiotics or phytogenic feed additives (PFAs) are widely investigated to evaluate their influence on improving gut health, increasing digestibility, and thereby the growth performance. The present study is a continuity of our experiments on dietary inclusion of Piper betle and Persicaria odorata leaf meal and the first of its kind to evaluate the comparative efficacy of phytobiotics (Piper betle and Persicaria odorata leaf meal), with halquinol and tetracycline in broiler chickens. The current experiment findings indicated that, in comparison with the control group, either of the dietary treatments positively modulated the gut morphology, improved ileal digestibility, maintained the intestinal population of Lactobacillus and reduced the pathogenic bacteria such as Staphylococcus aureus, Salmonella, Escherichia coli, and Clostridium spp., thus improved the growth performance in broiler chickens. Abstract The current experiment was designed to estimate the comparative efficacy of selected phytobiotics Persicaria odorata leaf meal (POLM) and Piper betle leaf meal (PBLM) with halquinol, and tetracycline in broiler chickens. The 150-day-old broiler chickens were randomly assigned to five dietary groups. The dietary supplementation groups were the basal diet (BD), which served as the negative control (NC), and BD + 0.2 g/kg tetracycline, which served as the positive control (PC); BD + 0.03 g/kg halquinol (HAL), BD + 8 g/kg POLM (Po8), and BD + 4 g/kg PBLM (Pb4) were the treatment groups. Growth performance, gut morphology, ileal digestibility, and cecal microbiota composition were measured. On day 21, the body weight gain (BWG) was enhanced (p < 0.05) in the broiler chickens fed on phytobiotics (Po8 and Pb4) relative to the NC group, however, on day 42 and in terms of overall growth performance, BWG was enhanced (p < 0.05 in diets (Po8, Pb4, HAL and PC) in comparison with the NC group. Conversely, feed conversion ratio (FCR) was recorded reduced (p < 0.05) in Pb4, Po8, HAL, and PC group in comparison with the NC group. Supplementation of phytobiotics (Po8 and Pb4), HAL and PC, positively improved the gut morphology compared to the NC group. Furthermore, the maximum (p < 0.05) villus height (VH) in duodenum and jejunum was observed in broilers fed on diet Pb4. Supplementation of phytobiotics, HAL and PC, improved (p < 0.05) the digestibility of dry matter (DM) (except for HAL), organic matter (OM), crude protein (CP), ether extract (EE), and ash compared to the NC group. Dietary supplementation of phytobiotics (Po8 and Pb4), HAL and PC, significantly reduced the E. coli, Salmonella, and Staphylococcus aureus (except for HAL) counts compared to the NC group. However, supplementation of Pb4 resulted in significantly decreased total anaerobic bacteria and Clostridium spp. counts compared to the NC group. In addition, supplementation of phytobiotics significantly increased the Lactobacillus count compared to HAL, PC, and NC groups. In conclusion, dietary supplementation of phytobiotics improved the gut morphology, positively modulated and maintained the dynamics of cecal microbiota with enhanced nutrient digestibility, thus, increased the growth performance. Based on current results, phytobiotics could be used as an alternative to AGPs for sustainable broiler chicken production.

Abstract licence: CC BY 4.0