Squill liquid extract 73mg/g / Ipecacuanha liquid extract 36mg/g pastilles

Osteoarthritis (OA) of the knee is a major health problem in the society. Iranian Traditional Medicine (ITM) or Persian Medicine (PM) as a branch of complementary medicine has been practiced in Iran for many centuries. An herbal medication known as squill oxymel has been used by PM physicians for OA. Our aim is to investigate the effect of squill oxymel on OA of the knee joint. Eighty eight patients were assigned to receive a placebo or squill oxymel syrup (10 ml each morning on empty stomach) for 8 consecutive weeks. Acetaminophen tablets were considered as the rescue medicine. Ultimately, 43 patients in the placebo group and 40 patients in the treatment group completed the trial and were included in the statistical analysis. Patients were followed for 4 weeks after cessation of treatment. The Knee injury and Osteoarthritis Outcome Score (KOOS) questionnaire and Visual Analog Scale (VAS) were considered as the main outcome measures. Laboratory tests including AST, ALT, BUN, Cr plus inflammatory tests including WBC, ESR, and CRP with specific tests i.e. interleukin 6 (IL6) and superoxide dismutase (SOD) at the beginning and the end of intervention were measured. The results showed the positive effect of treatment on the outcome of knee pain (p=0.04) and daily activity (p=0.01) of KOOS after Cessation of treatment. On the other hand, VAS decreased in both treatment and placebo groups; while it showed significance intra-group and showed no significance between the two groups. After 4 weeks of cessation of treatment, the positive effect of the squill oxymel on the treatment group continued in some of the subscales of KOOS, including symptoms, knee pain and daily activities, but stopped in the placebo group. In general, both clinically and statistically significant improvement was observed after cessation of treatment. Squill oxymel syrup showed promising results in management of knee OA but future researches with larger sample size and longer duration are necessary.

Abstract licence: CC BY-NC

Background: Carapichea ipecacuanha (Brot.) L. Andersson is the botanical source of Ipecac drug and contains major alkaloids emetine, cephaline that are pharmaceutically used against bronchitis associated with cough in children, severe diarrhea (amoebic dysentery) and also cancer. Ipecac serves as an expectorant to thin mucous and easy coughing. Low doses are used to enhance appetite and it is administered orally to cause vomiting after suspected poisoning. Materials and Methods: The review highlights the taxonomy, nomenclature, distribution, medicinal uses and major pharmacological activities including side effects of Ipecac drug reported in recent years consulting various published papers dealing with Ipecac. Results and Conclusion: The species is rarely distributed due to disturbances in their habitats in natural growing condition. Further studies are required to scientifically evaluate the traditional uses of this plant through extraction and identification of their active ingredients and the mechanisms and mode of action that would serve as a source of collective information on this plant.

Abstract licence: CC BY-NC-ND

Abstract Medicinal herbs are the main source of bioactive compounds used in the medical industry. White squill ( Urginea maritima ) is an important medicinal and ornamental plant cultivated in the Mediterranean region. This study reports an efficient protocol for in vitro propagation of Urginea and investigates important bioactive compounds present in the bulbs and in vitro –produced callus. The least number of days for callus induction and shoot regeneration was achieved with Murashige and Skoog (MS) basal media supplemented with 1.0 mg L −1 1-naphthaleneacetic acid (NAA) plus 0.1 mg L −1 6-benzylaminopurine (BAP) and 1.0 mg L −1 NAA plus 0.4 mg L −1 BAP, respectively, while the highest number of shoots and fresh weight were obtained at medium supplemented with 1.0 mg L −1 NAA plus 0.5 mg L −1 BAP. Gas chromatography-mass spectrometry (GC–MS) analysis of Urginea bulb methanol extract showed the existence of important secondary metabolites, such as palmitic acid (C 16 H 32 O 2 ), 9-hexadecenoic acid (C 16 H 30 O 2 ), phthalic acid 2-ethylhexyl propyl ester (C 19 H 28 O 4 ), tetradecanoic acid (C 14 H 28 O 2 ), undecanoic acid (C 11 H 22 O 2 ), and oleic acid (C 18 H 34 O 2 ), in addition to other important compounds, such as 13-heptadecyn-1-ol, 9,12-octadecadienoic acid, 1-monolinoleoylglycerol trimethylsilyl ether, 2-methyl-1-hexadecanol, and octadecanoic acid. Callus methanol extracts showed a reduction in the percentages of most phyto-components compared to bulb extract except for oleic acid, 3-(octadecyloxy) propyl ester and 3-hydroxydodecanoic acid; on the other hand, some important compounds were detected only in callus extract possessing anti-cancer, antiviral, and anti-inflammatory effects, such as farnesol (C 15 H 26 O), 7-methyl-Z-tetradecen-1-ol acetate (C 17 H 32 O 2 ), ethyl iso-allocholate (C 26 H 44 O 5 ), 4-trifluoroacetoxypentadecane (C 17 H 31 F 3 O 2 ), and 2-hydroxyhexadecanoic acid (C 16 H 32 O 3 ).

Abstract licence: CC BY

Urginea maritima L. (squill) species is widely spread at the Mediterranean region as two main varieties, i.e., white squill (WS) and red squill (RS), that are recognized for several health potentials. The major secondary metabolite classes of the squill are cardiac glycosides, mainly, bufadienolides, flavonoids, and anthocyanins. Herein, a multiplex MS and NMR metabolomics approach targeting secondary and aroma compounds in WS and RS was employed for varieties classification. Solid-phase micro extraction-gas chromatography/mass spectroscopy (SPME-GC/MS), ultra-high-performance liquid chromatography/mass spectrometry (UPLC/MS), as well as nuclear magnetic resonance (NMR) provided fingerprinting and structural confirmation of the major metabolites for both types of the squill. For comparison of the different platforms’ classification potential, multivariate data analysis was employed. While Bufadienolides, viz. “hydroxy-scilliglaucosidin-O-rhamnoside, desacetylscillirosidin-O-rhamnoside and bufotalidin-O-hexoside” as well as oxylipids, were enriched in WS, flavonoids, i.e., dihydro-kaempferol-O-hexoside and its aglycon, taxifolin derivative, were predominant in RS. A cytotoxicity screening against three cancer cell lines, including breast adenocarcinoma (MCF-7), lung (A-549), and ovarian (SKOV-3) cell lines was conducted. Results revealed that WS was more effective on A-549 and SKOV-3 cell lines (WS IC50 0.11 and 0.4 µg/mL, respectively) owing to its abundance of bufadienolides, while RS recorded IC50 (MCF7 cell line) 0.17 µg/mL since is is rich inflavonoids.

Abstract licence: CC BY

Database Management Systems (DBMSs) are the core of management information systems. Thus, detecting security bugs or vulnerabilities of DBMSs is an essential task. In recent years, grey-box fuzzing has been adopted to detect DBMS bugs for its high effectiveness. However, the seed scheduling strategy of existing fuzzing techniques does not consider the seeds’ correctness, which is inefficient in finding vulnerabilities in DBMSs. Moreover, current tools cannot correctly generate SQL statements with nested structures, which limits their effectiveness. This paper proposes a fuzzing solution named Squill to address these challenges. First, we propose correctness-guided mutation to utilize the correctness of seeds as feedback to guide fuzzing. Second, Squill embeds semantics-aware instantiation to correctly fill semantics to SQL statements with nested structures by collecting the context information of AST nodes. We implemented Squill based on Squirrel and evaluated it on three popular DBMSs: MySQL, MariaDB, and OceanBase. In our experiment, Squill explored 29% more paths and found 3.4× more bugs than the existing tool. In total, Squill detected 30 bugs in MySQL, 27 in MariaDB, and 6 in OceanBase. Overall, 19 of the bugs are fixed with 9 CVEs assigned. The results show that Squill outperforms the previous fuzzer in terms of both code coverage and bug discovery.

Abstract licence: CC BY

Cyclotides are head-to-tail cyclized peptides with a unique cystine-knot motif. Their structure provides exceptional resistance against enzymatic, chemical, or thermal degradation compared to other peptides. Peptide-based therapeutics promise high specificity, selectivity and lower immunogenicity, making them safer alternatives to small molecules or large biologicals. Cyclotides were researched due to their anti-cancer properties by inducing apoptosis in tumor cells in the past, but the impact of cyclotides on cytotoxic immune cells was poorly studied. Natural Killer (NK) cells are cytotoxic innate lymphoid cells and play an important role in the defense against infected, stressed and transformed cells. NK cells do not need prior sensitization and act in an antigen independent manner, holding promising potential in the field of immunotherapy. To investigate the effect of immunomodulatory cyclotides on NK cells, we evaluated several peptide-enriched plant extracts on NK cell mediated cytotoxicity. We observed that the extract samples derived from Carapichea ipecacuanha (Brot.) L. Andersson augments the killing potential of mouse NK cells against different tumor targets in vitro. Subsequent isolation of cyclotides from C. ipecacuanha extracts led to the identification of a primary candidate that enhances cytotoxicity of both mouse and human NK cells. The augmented killing is facilitated by the increased degranulation capacity of NK cells. In addition, we noted a direct toxic effect of caripe 8 on tumor cells, suggesting a dual therapeutic potential in cancer treatment. This study offers novel insights how natural peptides can influence NK cell cytotoxicity. These pre-clinical findings hold significant promise for advancing current immunotherapeutic approaches.

Abstract licence: CC BY

Two bulbous plant species, Squilla maura and Oncostema peruviana (Asparagaceae), show particularly interesting ornamental and medicinal characteristics. Micropropagation could be a promising alternative method to accelerate their naturally slow spreading pattern. This study focused on the effects of different growth regulators and culture media on callus induction and shoot regeneration, from which an effective protocol was established. Leaf explants were cultured on Murashige and Skoog (MS), MS/2, or B5 medium, containing one auxin—1-Naphthaleneacetic acid (NAA), 2,4-Dichlorophenoxyacetic acid (2.4-D), or 3-indolebutyric acid (IBA)—combined with the cytokinin 6-Benzylaminopurine (BAP), at various concentrations. All treatments resulted in callogenesis rates between 65–100% for both species. These treatments led to direct bulbil regeneration. Among the treatments, the B5 medium with 1 mgL−1 BAP and 1 mgL−1 2,4-D gave the highest regeneration rate (89.2%) for O. peruviana, while the ½MS medium with 0.5 mgL−1 BAP and 0.5 mgL−1 NAA showed the highest regeneration rate (85.5%) for S. maura. The highest mean number of bulils per explant was 7.44 for O. peruviana on the MS medium (0.5 mgL−1 BAP and 2 mgL−1 IBA), and 9.5 for S. maura on the MS medium (1 mgL−1 BAP and 0.5 mgL−1 NAA). The regenerated bulbils were transferred for multiplication to the MS medium with a hormone combination (2 mgL−1 BAP and 0.2 mgL−1 NAA) which increased the multiplication rate compared to the callus induction me dium, with a highest recorded multiplication rate of 177 (O. peruviana) and 104.33 (S. maura). The propagation stage achieved the highest number of bulbils/explant after a second subculture for the two species. An efficient micropropagation protocol was established for S. maura, which answers our main objective, and it would contribute to their conservation and sustainable use in ornamental and medicinal applications.

Abstract licence: CC BY