Diloxanide 50mg/5ml oral solution

Diloxanide furoate (DXF) and Metronidazole (MTZ) are commonly combined as a binary mixture for the treatment of a variety of diseases brought on by bacteria and parasites including amoebiasis and giardiasis. Different analytical methods were established for determination of both DXF and MTZ, However, only one chemometric spectrophotometric method was established for their stability indicating determination which involves problematical steps and specific software program. Herein, double divisor ratio spectra derivative (DDRSD) method is exploited for quantification of DXF and MTZ in presence of DXF-degradation products (FUR and DEG). The recommended DDRSD method is linear over the range of 2–25 and 1–25 µg/mL for DXF and MTZ, correspondingly. Additionally, the suggested method was effectively employed to their pharmaceutical formulations with good findings. Besides, the recommended DDRSD method was validated conforming to ICH guidelines. The introduced DDRSD method is simple, selective, and economic and could be implemented for quality control samples of the studied drugs.

Abstract licence: CC BY 4.0

Abstract Objective : To develop and validate a simple, precise, and stability-indicating reversed-phase high-performance liquid chromatographic (RP-HPLC) method for the simultaneous determination of metronidazole benzoate, diloxanide furoate, methylparaben, and propylparaben in a combined pharmaceutical suspension. Results : Chromatographic separation was achieved on a C18 column (4.6 × 150 mm, 5 µm) using an isocratic mobile phase consisting of ammonium acetate buffer (pH 7.0), methanol, and acetonitrile, with photodiode array detection at 254 nm. All analytes were well resolved within 12 minutes. The method was validated according to ICH Q2(R1), GEON, and U.S. FDA ORA guidelines. It demonstrated excellent linearity (R² > 0.999), satisfactory accuracy (recoveries within acceptable limits), and high precision (%RSD ≤ 1.1%). The limits of detection and quantification confirmed adequate sensitivity. The method was found to be robust and specific, with no interference from excipients or degradation products under stress conditions. This validated method is suitable for routine quality control and stability testing of pharmaceutical formulations containing these compounds.

Abstract licence: CC BY 4.0

We examined the case report written by Ke et al, describing a rare clinical case. In this editorial, we would like to emphasize the differential diagnosis of rectal masses through a rare case. We describe a case of ameboma, which manifested itself as a mass in the rectum in terms of imaging and rectoscopic features, in an immunocompetent patient who had complaints of constipation and rectal bleeding for weeks. The initial diagnosis suggested malignancy due to imaging and rectoscopic features, but the pathology report reported it as amoebiasis. After ten days of metronidazole and oral amebicide (diloxanide furoate) treatment, the patient's symptoms and radiological findings were successfully regressed.

Abstract licence: CC BY-NC

A new method was established for simultaneous estimation of Tinidazole and Diloxanide furoate by RP-HPLC method. The chromatographic conditions were successfully developed for the separation of Tinidazole and Diloxanide furoate by using Thermosil RP C18 (4.5×100 mm) 5.0µm, flow rate was 1ml/min, mobile phase ratio was (70:30 v/v) methanol. The retention times were found to be 2.408mins and 3.016mins. The % purity of Tinidazole and Diloxanide furoate was found to be 99.24% and 101.27% respectively. The system suitability parameters for Tinidazole and Diloxanide furoate such as theoretical plates and tailing factor were found to be 4668, 1.3 and 6089 and 1.2, the resolution was found to be 6.2. The analytical method was validated according to ICH guidelines (ICH, Q2 (R1)). The linearity study Tinidazole and Diloxanide furoate was found in concentration range of 50ppm-250ppm and 5ppm-25ppm and correlation coefficient (r2) was found to be 0.999 and 0.999, % recovery was found to be 100.56% and 101.47%, %RSD for repeatability was 0.1 and 0.3, % RSD for intermediate precision was 0.19 and 0.57respectively. The precision study was precise, robust, and repeatable. LOD value was 4.27 and 6.80, and LOQ value was 0.0272 and 0.3125 respectively.

Abstract licence: CC BY-NC 4.0