Clobetasol 500microgram / Neomycin 5mg / Nystatin 100,000units/g ointment

Neomycin sulphate is official in IP 2018, USP 2004 and BP 2003; which includes Microbial assay (Cylinder Plate assay) for the estimation therefore chemical derivatization of Neomycin Sulphate is done using 1-Fluoro-2, 4-Dinitrobenzene (DNFB); also known as Sanger’s reagent, to make it detectable by UV with good accuracy and precision. A fixed dose combination of Neomycin Sulphate, Clobetasol Propionate and Chlorocresol (preservative) in cream formulation is used to treat different types of Skin infections. The literature review reveals that methods like UV, HPLC, HPTLC, GC-MS etc. have been reported for Neomycin Sulphate and Clobetasol Propionate individually and along with other drugs. But no reported method found in combination for Neomycin sulphate and Clobetasol propionate. A simple, accurate, precise and economical UV spectrophotometric i.e. Area Under Curve Method (AUC) was developed. All the dilutions of drugs were prepared in Methanol: Acetonitrile (50:50). Area under curve was integrated in the wavelength range of 235.0- 245.0 nm, 252.0-262.0 nm and 223.0-233.0 nm for CSP, NMS and CCS respectively. The AUC method for Clobetasol Propionate (CSP), Neomycin Sulphate (NMS) and Chlorocresol (CCS) was found to be linear over the range of 0.8-1.2 μg/ml, 8.0-12.0 μg/ml and 1.6-2.4 μg/ml respectively. The result of analysis was analysed and validated statistically and recovery study was found within range of 98-102%. The % RSD was not more than 2.0 % which indicates good precision. All the validation parameters were carried out according to ICH Q2 (R1) guidelines.

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Background/Objectives: Aerobic vaginitis (AV) and bacterial vaginosis (BV) are vaginal infections requiring the fast elimination of pathogens. The frequent confusion of these infections may justify the use of a rapidly acting broad-spectrum antibiotic treatment. Methods: This study investigated the bactericidal kinetics of the neomycin-polymyxin B-nystatin (NPN) combination compared to those of two reference antibiotics (clindamycin and metronidazole) against 22 bacteria commonly implicated in AV and BV. Results: NPN exhibited bactericidal activity against the aerobic Gram-positive bacteria, with particularly high bactericidal activity being observed against streptococci, S. aureus, and C. amycolatum after 1 h at low dilutions and after 4 h for all dilutions. Enterococci were less sensitive to NPN. Clindamycin demonstrated poor rapid bactericidal activity against all Gram-positive bacteria tested. NPN manifested high bactericidal activity against all aerobic Gram-negative bacteria tested, whereas clindamycin showed bactericidal activity only after 4 h at a 1/2 dilution. With respect to the four anaerobic strains tested, NPN demonstrated high bactericidal activity at all tested dilutions with concentration-dependent effects. Metronidazole exhibited lower or no rapid bactericidal activity. Conclusions: These results suggest that NPN has very fast bactericidal action against the main bacteria involved in AV and BV compared to clindamycin and metronidazole, highlighting its potential in managing bacterial vaginal infections.

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Schiff bases compounds were synthesized by reaction of the Benzidine with different aldehydes and ketones by using microwave method to obtain compounds (N1-N5). Thiazolidine 4-one compounds were prepared by the cyclization of Schiff bases with thioglycolic acid to obtain thiazolidine 4-one compounds (N6-N10). The prepared compounds were characterized by physical methods, through melting points and color, as well as by spectroscopic methods such as FT-IR and 1H-NMR. The purity of the prepared compounds was evaluated using TLC. The bioactivity of these compounds was tested on the growth of one type of a fungus of the yeast variety Candida was studied and type of bacterial isolates of Bacillus pumilus and the standard fungicide (Nystatin) of the fungus was used and the standard antibiotic (neomycin sulfate) of bacteria and the results indicate that the synthesized compounds have the ability to inhibit the fungus and bacteria used by using different concentrations. Molecular docking studies were conducted to examine how some of the synthesized compounds bind to the putative target, Protein structures i.e. HER2 (PDB ID: 1N8Z), Carcinoembryonic antigen (PDB ID; 2VER), BRCA1 (PDB ID 4OFB), BRCA2 (PDB ID 1MJE).

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The aim of study is production of new local drug consisting of Nystatin, Neomycin sulfate, Dexamethasone ointment are veterinary medicinal preparations used in the treatment of exogenous fungi and infections in small and large animals that cause by (candida albicans) and affected by gram positive and gram negative bacteria like (Staphylococcus aureus, Escherichia coli, Haemophilus influenza, Klebsiella-Enterobacter species, Neisseria species, and Pseudomonas aeruginosa). Nystatin is considered one of the safe drugs when treating external fungal infections in field animals. Neomycin works by inhibiting protein synthesis in the bacterial cell and thus leads to the killing of bacteria either dexamethasone works to stop infections in the affected area of ​​animals. In order for this product to be both research and applied, many of the initial pharmaceutical compositions were prepared until the final and stable composition was reached in this form. (90-110% permissible limit of activity). This process included several stages of collecting information on the substances included in the formula, active substances and additives, and then preparing the formula according to the specifications. International pharmacology using pharmacopoeia, followed by a study of stability and resistance of the preparation at room temperatures, then sending samples for field examination to the veterinary department and for the lack of cases for other animals where it was used on cases of fungal infections and skin infections in small animals (Cats). The treatment period was 5-10 days, and it brought very good results, according to the field evaluation form attached to the research. Outcomes of study its good and new research and very important to treatment skin infection in small animals.

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Background: Acute otitis externa (AOE) is a common condition with multiple available treatments. This study aimed to compare gauze strips soaked in triamcinolone, neomycin, and nystatin with conventional eardrops for the treatment of AOE. Methods: This experimental study included patients with AOE referred to the Otolaryngology Clinic of Shahid Mohammadi Hospital, Bandar Abbas, Iran, 2014-2015. Based on their signs and symptoms, patients were divided into two clinical groups of mild to moderate and moderate to severe AOE. Patients treated with polymyxin B, neomycin, and hydrocortisone (polymyxin NH), or ciprofloxacin plus betamethasone eardrops were regarded as controls (conventional eardrops), and those whose ear canal had been filled with a gauze strip soaked in triamcinolone, neomycin, and nystatin (triamcinolone NN) were regarded as cases. Results: A total of 76 ears were included in this study (36 and 40 cases with mild to moderate and moderate to severe AOE, respectively). After 24 hours, in both clinical groups with mild to moderate and moderate to severe AOE, response to treatment with triamcinolone NN-soaked gauze strips was significantly higher than conventional eardrops (94.4% vs. 11.1%, P<0.001 and 80% vs. 10%, P<0.001, respectively). Conclusion: Patients with mild to severe AOE appear to rapidly respond to gauze strips soaked in triamcinolone, neomycin, and nystatin.

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Background. Atopobium vaginae is an important component of the complex abnormal anaerobic vaginal microflora in vaginal dysbiosis and is believed to be at least partially responsible for known adverse gynecological and obstetric outcomes. Resistance of A. vaginae to traditional therapy (metronidazole and clindamycin) is described.Objective of the study: to evaluate the effectiveness of topical therapy with the combined drug ternidazole–neomycin sulfate–nystatin–prednisoloneof vaginal dysbiosis associated with A. vaginae in diagnostically significant concentrations in non-pregnant and pregnant women of reproductive age with a history of reproductive failure.Materials and methods. 78 women of reproductive age with vaginal dysbiosis associated with A. vaginae in diagnostically significant concentrations were under observation. 37 infertile women had recurrent implantation failures and 41 pregnant women with infertility treated in cycles of assisted reproductive technologies. Control groups consisted of 30 fertile non-pregnant women and 30 pregnant women after natural conception with vaginal normocenosis. Comprehensive quantitative assessment of the vaginal anaerobic microbiota was performed using real-time polymerase chain reaction.Results. A. vaginae in diagnostically significant concentrations in the vaginal microbiota in the vast majority of cases is accompanied by the presence of Gardnerella vaginalis and other co-pathogens, mixed infections. Every second woman has aerobic-anaerobic dysbiosis, which makes it advisable to use combined topical drugs. The use of ternidazole–neomycin sulfate–nystatin–prednisolone vaginal tablets resulted in the absence of facultative and obligate anaerobes, Ureaplasma (urealiticum + parvum) and Candida spp. in the vaginal microbiota in diagnostically significant concentrations in women of treated groups. The average relative content of none of the studied conditionally pathogenic microorganisms after treatment did not exceed -3 CFU.Conclusions. The combined vaginal drug ternidazole–neomycin sulfate–nystatin–prednisolone is a highly effective, safe and compliant remedy for restoring vaginal health in non-pregnant and pregnant women with a history of reproductive failure and vaginal dysbiosis associated with the presence of A. vaginae in diagnostically significant concentrations.

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Aerobic vaginitis (AV) is an inflammatory disease of the vagina that occurs as a result of dysbiosis of the vaginal environment with a predominance of aerobic opportunistic microorganisms, such as Escherichia coli, Staphylococcus aureus, Streptococcus spp., etc. Frequent recurrence and insufficient diagnosis determine the relevance of research into effective methods of therapy for this disease.The objective: to compare the clinical and microbiological efficacy of two vaginal combination preparations in the treatment of AV in non-pregnant women.Materials and methods. The study included 80 non-pregnant women aged 18 to 45 years with a confirmed diagnosis of AV. The patients were randomized into two equal groups: the main group received medication Tergynan (ternidazole, neomycin sulfate, nystatin, prednisolone sodium metasulfobenzoate), and the comparison group received another combination preparation (ornidazole, miconazole, neomycin sulfate, prednisolone). Diagnostics included microscopy, bacteriological examination of vaginal discharge, gynecological examination, and a patient questionnaire. Treatment effectiveness was evaluated on the 11th and 30th days after therapy.Results. Patients in the main group experienced faster clinical symptom relief (1–2 days earlier) and the frequency of complete recovery immediately after the end of therapy was 85% versus 62% in the comparison group. After 30 days, recurrence occurred in only 9% of women who used Tergynan versus 36% in the comparison group.Conclusions. Tergynan demonstrated significantly higher clinical and microbiological efficacy in the treatment of AV, which justifies its advantages as the drug of choice in the case of this pathology.

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Restoration of the vaginal microbiocenosis is a mandatory component of the complex of therapeutic and preventive measures in women carrying a pregnancy under martial law.The objective: to study the role of synbiotics in the treatment of vaginal microbiocenosis disorders during pregnancy in military personnel and temporarily displaced women under martial law.Materials and methods. In total, 96 pregnant women aged 19 to 44 years with diagnosis vaginitis and vaginosis were examined and treated in the II and III trimesters: I group – 31 pregnant military women; II group – 33 pregnant women who were displaced from the combat zones (Zaporizhzhya, Kherson, Kharkov, Donetsk and Lugansk regions); III group – 32 pregnant women living in Kyiv and Kyiv region and who do not serve in Armed Forces of Ukraine (AFU). For the treatment of vaginitis and vaginosis in pregnant women of these three groups, according to the identified pathogens, a drug containing ornidazole – 500 mg, neomycin sulfate – 100 mg, nystatin – 100,000 IU, prednisolone – 3 mg was used intravaginally for 10 days. In addition, for pregnant women in the I and II groups a drug containing a combination of probiotics and prebiotics, a synbiotic, was included in the treatment regimen to correct vaginal microflora disorders.Results. During the examination, normal vaginal pH was detected only in 3 (9.7%) women in the I group and 4 (12.1%) pregnant women in the II group, which necessitated the mandatory use of synbiotics in the treatment schemes.Microscopy data revealed high fungal-bacterial contamination of the vaginal microflora in women of the I and II groups, namely:– gram-positive flora (bacilli – in 14 (45.2%) cases of the I group and 16 (48.5%) of the II group; cocci – in 12 (38.7%) and 14 (42.4%), respectively);– gram-negative flora (bacilli – in 10 (32.2%) and 12 (36.4%); cocci – in 14 (45.2%) and 13 (39.4%);– extracellular diplococci – in 9 (29.0%) and 12 (36.4%);– “key” cells – in 9 (29.0%) and 10 (30.3%);– fungi of the genus Candida – in 23 (74.2%) and 20 (60.6%) women, respectively.The obtained results of molecular diagnostics confirmed the microscopy data: Gardnerella vaginalis was found in 9 (29.0%) and 11 (30.3%) women of the I and II groups, Atopobium vaginae – in 5 (16.1%) and 6 (18.2%), Mobiluncus spp. – in 7 (22.6%) and 6 (18.2%) women, respectively.Conclusions. The study of the vaginal microbiocenosis and subsequent treatment of vaginitis and vaginosis in pregnant women demonstrated that the normalization of vaginal pH after treatment was significantly better in pregnant servicewomen and displaced women, who received symbiotic in addition to vaginal tablets with ornidazole, neomycin sulfate, nystatin, prednisolone – in 90.3% and 90.9% of patients, respectively. In the III group (residents of Kyiv and Kyiv region who do not serve in the AFU), normalization of vaginal pH was observed only in 65.6% of patients.Molecular diagnostic data by groups before and after treatment of vaginitis and vaginosis indicate that in pregnant servicewomen and displaced women, the beneficial microflora Lactobacillus spp. recovered significantly better – from 9.7 to 93.6% in women of the I group, from 12.1 to 93.9% in the II group, than in pregnant women of the III group – from 46.9 to 68.8%. Pathogenic microflora (Gardnerella vaginalis, Atopobium vaginae, Mobiluncus spp., Bacteroides fragilis, Megasphaera type 1) was not detected after treatment in all three groups.The use of a new generation drug – a synbiotic that combines a probiotic and a prebiotic, opens up new opportunities for the effective treatment of vaginitis and vaginal vaginosis in pregnant servicewomen and internally displaced women.

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