Belladonna medicated plaster 28cm x 17.5cm

The name Atropa is thought to have been derived from the Greek goddess Atropos while, meaning a beautiful lady. Belladonna has also been used over the past centuries to relax muscles, especially of the stomach and intestines, to help relieve intestinal colic, and to treat peptic ulcers. It also relaxes the urinary tract, removing spasms. The ancients used the plant to treat Parkinson's disease, as it works to reduce tremors and stiffness. Modern medicine proved that belladonna alkaloids inhibit the parasympathetic central nervous system that controls the various involuntary activities of the body. These alkaloids increase heartbeats. It has been shown that belladonna is a sedative used to remove the pain of diseases accompanied by bouts of muscle contractions.

Abstract licence: CC BY

Atropa belladonna ( A. belladonna ), commonly known as deadly nightshade, is a poisonous plant belonging to the Solanaceae family. The toxic effects of A. belladonna are attributable to its alkaloid content, which possesses potent anticholinergic properties. These alkaloids are responsible for the plant’s toxicity and can cause a range of adverse effects in humans and animals upon ingestion or contact. In this report, we describe two atypical cases of A. belladonna poisoning resulting from accidental ingestion of the plant’s raw leaves, which were referred to the emergency room of a poisoning center in northern Iran. Both patients presented with symptoms of anticholinergic toxicity, including dry mouth, mydriasis, tachycardia, and delirium. The patients were managed conservatively with supportive measures, including hydration and administration of benzodiazepines to control agitation and delirium. With appropriate treatment, both patients showed improvement and were discharged from the hospital. A. belladonna intoxication is associated with a range of clinical manifestations, primarily due to its neurotoxic effects. These manifestations may include flushing, mydriasis, tachycardia, ataxia, agitation, delirium, and urinary retention. The severity of symptoms can vary depending on the amount of the toxin ingested and the individual’s susceptibility. In severe cases, A. belladonna toxicity can lead to seizures, coma, and even death. These cases highlight the importance of awareness regarding the potential toxicity of A. belladonna and the necessity of prompt and appropriate management of its toxicity. In severe cases, physostigmine may be considered for the treatment of neurological symptoms due to the plant’s anticholinergic effects.

Abstract licence: CC BY

Anthocyanins are water-soluble flavonoid pigments that play a crucial role in plant growth and metabolism. They serve as attractants for animals by providing plants with red, blue, and purple pigments, facilitating pollination and seed dispersal. The fruits of solanaceous plants, tomato (Solanum lycopersicum) and eggplant (Solanum melongena), primarily accumulate anthocyanins in the fruit peels, while the ripe fruits of Atropa belladonna (Ab) have a dark purple flesh due to anthocyanin accumulation. In this study, an R2R3-MYB transcription factor (TF), AbMYB1, was identified through association analysis of gene expression and anthocyanin accumulation in different tissues of A. belladonna. Its role in regulating anthocyanin biosynthesis was investigated through gene overexpression and RNA interference (RNAi). Overexpression of AbMYB1 significantly enhanced the expression of anthocyanin biosynthesis genes, such as AbF3H, AbF3′5′H, AbDFR, AbANS, and Ab3GT, leading to increased anthocyanin production. Conversely, RNAi-mediated suppression of AbMYB1 resulted in decreased expression of most anthocyanin biosynthesis genes, as well as reduced anthocyanin contents in A. belladonna. Overall, AbMYB1 was identified as a fruit-expressed R2R3-MYB TF that positively regulated anthocyanin biosynthesis in A. belladonna. This study provides valuable insights into the regulation of anthocyanin biosynthesis in Solanaceae plants, laying the foundation for understanding anthocyanin accumulation especially in the whole fruits of solanaceous plants.

Abstract licence: CC BY

: In the present work, the chemical composition of extract fractions of the Atropa belladonna plant growing in the north of Iran was investigated by HPTLC, HPLC, and GC-MS. Based on HPLC results, atropine, and scopolamine were found to be higher in the fruit and leaf extracts than in other parts of the plant. The comparative GC-MS analysis showed that diacetone alcohol, mesityl oxide, palmitic acid, and linoleic acid were the major bioactive components in the root, stem, leaf, and fruit extracts, respectively. Leaf extract showed the best antioxidant activity in the DPPH test. The antibacterial activity of fractional extracts was determined against Pseudomonas aeruginosa using the MIC method, and the fruit and leaf extracts exhibited the best antibacterial activities. The leaf extract was embedded into nanofibers by electrospinning technique, and its antibacterial activity was determined against Pseudomonas aeruginosa. The morphology and mechanical properties of the nanofibers were studied with SEM, contact angle, and tensile analysis, showing ultrafine fibers with uniform morphology.

Abstract licence: CC BY-NC